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Adaptive optics lattice light-sheet imaging and AI powered big data processing of live stem cell-derived

Biomaterial-Pushed Immunomodulation: Cell Biology-Primarily based Methods to Mitigate Extreme Irritation and Sepsis

 

Irritation is an integral part of all kinds of illness processes and oftentimes can enhance the deleterious results of a illness. Discovering methods to modulate this important immune course of is the idea for a lot of therapeutics below improvement and is a burgeoning space of analysis for each primary and translational immunology.

Along with growing therapeutics for mobile and molecular targets, the usage of biomaterials to change innate and adaptive immune responses is an space that has just lately sparked vital curiosity. Specifically, immunomodulatory exercise may be engineered into biomaterials to elicit heightened or dampened immune responses to be used in vaccines, immune tolerance, or anti-inflammatory purposes.

Importantly, the inherent physicochemical properties of the biomaterials play a major function in figuring out the noticed results. Properties together with composition, molecular weight, dimension, floor cost, and others have an effect on interactions with immune cells (i.e., nano-bio interactions) and permit for differential organic responses reminiscent of activation or inhibition of inflammatory signaling pathways, floor molecule expression, and antigen presentation to be encoded.

Quite a few alternatives to open new avenues of analysis to grasp the methods wherein immune cells work together with and combine info from their surroundings might present vital options wanted to deal with a wide range of issues and illnesses the place immune dysregulation is a key inciting occasion. Nevertheless, to elicit predictable immune responses there’s a nice want for an intensive understanding of how the biomaterial properties may be tuned to harness a designed immunological consequence.

This assessment goals to systematically describe the organic results of nanoparticle properties-separate from extra small molecule or biologic delivery-on modulating innate immune cell responses within the context of extreme irritation and sepsis. We suggest that nanoparticles signify a possible polypharmacological technique to concurrently modify a number of points of dysregulated immune responses the place single goal therapies have fallen brief for these purposes.

This assessment intends to function a useful resource for immunology labs and different related fields that wish to apply the rising area of rationally designed biomaterials into their work.

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Porcine Parvovirus Antibody Elisa Test Kit

767-LSY-30009 192 Wells/kit
EUR 382

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

FERR ( Ferritin) ELISA test

7 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FERR ( Ferritin)

Syphilis Antibody ELISA test

100 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Syphilis Antibody

TSH (Thyrotrophin) ELISA test

14 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TSH (Thyrotrophin)

PRL (Prolactin) ELISA test

4 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PRL (Prolactin)

E2 (Estradiol) ELISA test

5 96T/Box Ask for price
Description: ELISA based test for quantitative detection of E2 (Estradiol)

PROG (Progesterone) ELISA test

20 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PROG (Progesterone)

TESTO (Testosterone) ELISA test

19 96T/Box Ask for price
Description: ELISA based test for quantitative detection of O (osterone)

T3 (Triiodothyronine) ELISA test

15 96T/Box Ask for price
Description: ELISA based test for quantitative detection of T3 (Triiodothyronine)

T4 (Thyroxine) ELISA test

16 96T/Box Ask for price
Description: ELISA based test for quantitative detection of T4 (Thyroxine)

CA72-4 ELISA test

24 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA72-4

HBcAb Sandwich ELISA test

95 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HBcAb Sandwich

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-20tests 20 tests
EUR 283.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Accu-Tell COVID-19 IgG/IgM Rapid Test

GEN-B352-40tests 40 tests
EUR 385.2
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

Melamine Rapid Test Kit

abx092011-50tests 50 tests
EUR 444

NDV rapid test kit

RG15-03 1 box
EUR 166.86
Description: Please check the datasheet of NDV rapid test kit before using the test.

Staphyloslide Latex Test Kit

240915 PK100
EUR 272.46

Hach Test Kit CYANIDE

LCK315 EACH
EUR 144.28

Hach Test Kit FLUORIDE

LCK323 EACH
EUR 136.62

Hach Test Kit COPPER

LCK329 EACH
EUR 178.75

Hach Test Kit NICKEL

LCK337 EACH
EUR 183.86

Hach Test Kit SULPHIDE

LCK653 EACH
EUR 163.43

Elga Chlorine Test Kit

WAT4098 EACH
EUR 385.32

?-HCG (Human chorionic gonadotropin) ELISA test

1 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ?-HCG (Human chorionic gonadotropin)

HGH (Human chorionic gonadotropin) ELISA test

22 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin)

NOVATest IgG/IgM Antibody Rapid Test Kit (NOVA Test)

UNCOV-40 40 Tests
EUR 115

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Test product

testtt 0 Ask for price

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Human toluidine Red Unheated Serum Test (TRUST) ELISA Kit

abx257506-96tests 96 tests
EUR 764.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

NOVATest Antigen Rapid Test Kit (For Single Use) (NOVA Test)

nCov-500-01 1T
EUR 162

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

FSH (Human Follicle-stimulating hormone) ELISA test

3 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

AFP (Alpha fetoprotein) ELISA test

6 96T/Box Ask for price
Description: ELISA based test for quantitative detection of AFP (Alpha fetoprotein)

CA199 (Cancer antigen) ELISA test

13 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA199 (Cancer antigen)

LH (Luteinizing Hormone) ELISA test

2 96T/Box Ask for price
Description: ELISA based test for quantitative detection of LH (Luteinizing Hormone)

CA153 (Cancer antigen) ELISA test

12 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA153 (Cancer antigen)

CA125 (Cancer antigen) ELISA test

11 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA125 (Cancer antigen)

CA50 (Cancer antigen) ELISA test

10 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CA50 (Cancer antigen)

FT4 (Free thyroxine) ELISA test

18 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FT4 (Free thyroxine)

FT3 (Free triiodothyronine) ELISA test

17 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine)

HEV antibody IgM ELISA test

92 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HEV antibody IgM

CEA (Cancer antigen) ELISA test

9 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CEA (Cancer antigen)

HAV antibody IgM ELISA test

75 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HAV antibody IgM

Human Echovirus (ECHO) IgM Rapid Test Kit

abx092093-20tests 20 tests
EUR 326.4

Human Toxoplasma (TOX) IgG Rapid Test Kit

abx092098-20tests 20 tests
EUR 260.4

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

Melamine (MEL) Rapid Test Kit

abx092057-50tests 50 tests
EUR 444

Clenbuterol (CLE) Rapid Test Kit

abx092058-50tests 50 tests
EUR 292.8

Ractopamine (RAC) Rapid Test Kit

abx092059-50tests 50 tests
EUR 292.8

Salbutamol (SAL) Rapid Test Kit

abx092060-50tests 50 tests
EUR 292.8

Tetracycline (TCs) Rapid Test Kit

abx092063-50tests 50 tests
EUR 477.6

Sulfonamides (Sas) Rapid Test Kit

abx092064-40tests 40 tests
EUR 477.6

Quinolones (QNs) Rapid Test Kit

abx092065-40tests 40 tests
EUR 477.6

Ciprofloxacin (CPFX) Rapid Test Kit

abx092066-50tests 50 tests
EUR 477.6

Quinolones (QNs) Rapid Test Kit

abx092067-40tests 40 tests
EUR 477.6

Brucella Antibody Rapid Test Kit

abx092069-40tests 40 tests
EUR 427.2

Antibody ISOTYPING TEST KIT (OKSA11303)

OKSA11303 10 Tests
EUR 936
Description: Description of target: MOUSE MONOCLONAL ANTIBODY ISOTYPING TEST KIT;Species reactivity: Mouse;Application: IS;Assay info: ;Sensitivity:

PCRAgH5 AIV Detection Test Kit

PD55-02 1 kit
EUR 1082.96
Description: Please check the datasheet of PCRAgH5 AIV Detection Test Kit before using the test.

Rapid Leishmania Ab Test Kit

RB2104 1 box
EUR 152.4
Description: Please check the datasheet of Rapid Leishmania Ab Test Kit before using the test.

Rapid PED Ag Test Kit

RG14-01 1 box
EUR 191.88
Description: Please check the datasheet of Rapid PED Ag Test Kit before using the test.

Thermal Test Film Starter Kit

PCR0892 EACH
EUR 275.88

Thermal Test Film Roll Kit

PCR0894 EACH
EUR 234.84

Hanna Test Kit Chlorine Free

WAT1572 EACH
EUR 30.78

Penicillin Drug Stability Test Kit

PST100 100 assays
EUR 231.22
Description: This product includes 0.5 ml of the 10 x PST dye for 100 assays using 96-well plates or micro-cuvettes.

Liposome Plasma Stability Test Kit

SPS20 20 columns
EUR 234.99
Description: This product includes 20 spin-columns for liposome stability studies in plasma

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

2019-nCoV IgG/IgM Rapid Test Cassette (Whole Blood/Serum/Plasma)

GEN-402-25tests 25 tests
EUR 292.8
Description: A rapid test for detection of antibodies (IgG and IgM) for 2019-nCoV, the novel Coronavirus from the Wuhan strain. The test is easy to perform, takes 10 minutes to provide reliable results and is higly specific to the 2019-nCoV Coronavirus.

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

DNASE TEST AGAR

D04-101-10kg 10 kg Ask for price

DNASE TEST AGAR

D04-101-2Kg 2 Kg Ask for price

DNASE TEST AGAR

D04-101-500g 500 g Ask for price

DERMATOPHYTE TEST AGAR

D04-107-10kg 10 kg
EUR 1018.8

DERMATOPHYTE TEST AGAR

D04-107-2Kg 2 Kg
EUR 268.8

DERMATOPHYTE TEST AGAR

D04-107-500g 500 g
EUR 116.4

MOTILITY TEST MEDIUM

M13-121-10kg 10 kg
EUR 1833.6

MOTILITY TEST MEDIUM

M13-121-2Kg 2 Kg
EUR 445.2

MOTILITY TEST MEDIUM

M13-121-500g 500 g
EUR 164.4

SNYDER TEST AGAR

S19-119-10kg 10 kg
EUR 1258.8

SNYDER TEST AGAR

S19-119-2Kg 2 Kg
EUR 320.4

SNYDER TEST AGAR

S19-119-500g 500 g
EUR 130.8

SAM Test Strip

TS00201s-30 30 tests
EUR 499.2
Description: S-adenosylmethionine quantitative test strip for serum, plasma and whole blood

SAH Test Strip

TS00301s-30 30 tests
EUR 604.8
Description: S-adenosylhomocysteine quantitative test strip for serum, plasma and whole blood

HCY Test Strip

TS00401s-30 30 tests
EUR 663.6
Description: Serum homocysteine quantitative test strip

Test Tube 16x100mm

3840/16X100 PK100
EUR 57.11

Dryslide Oxidase Test

231746 PK75
EUR 199.5

aa test product

123 1MG
EUR 279.6

Phenols Cuvette Test

LCK345 EACH
EUR 141.72

Dermatophyte Test Medium

MED1484 EACH
EUR 87.78

Test for Enteropathogenic.C

MIC6774 PK50
EUR 215.46

Sulphite Test Strips

MN91306 PK100
EUR 51.53

Thermal Test Film

PCR0890 PK25
EUR 383.04

5mL Test Tubes

TUB0228 PK1400
EUR 159.6

Spectroquant COD test

WAT1080 EACH
EUR 96.9

Phosphate Total Test

WAT3655 EACH
EUR 203.01

HPV (Human Papilloma Virus Antibody IgG) ELISA test

49 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HPV (Human Papilloma Virus Antibody IgG)

Human Hepatitis A Virus IgG Rapid Test Kit

abx092012-25tests 25 tests
EUR 510

Human Chlamydia Pneumoniae (CP) IgG Rapid Test Kit

abx092079-20tests 20 tests
EUR 309.6

Human Chlamydia Pneumoniae (CP) IgM Rapid Test Kit

abx092080-20tests 20 tests
EUR 309.6

Human Mycoplasma Pneumoniae (MP) IgG Rapid Test Kit

abx092081-20tests 20 tests
EUR 309.6

Human Mycoplasma Pneumoniae (MP) IgM Rapid Test Kit

abx092082-20tests 20 tests
EUR 309.6

Human Mycobacterium Tuberculosis (MT) IgG Rapid Test Kit

abx092084-20tests 20 tests
EUR 276

Human Helicobacter Pylori (HP) IgG Rapid Test Kit

abx092090-20tests 20 tests
EUR 276

Human Enterovirus 71 (EV71) IgM Rapid Test Kit

abx092091-20tests 20 tests
EUR 410.4

Human Hantavirus (HV) IgM / IgG Rapid Test Kit

abx092094-20tests 20 tests
EUR 326.4

Human Streptococcus Pneumoniae (SP) Antigen Rapid Test Kit

abx092096-20tests 20 tests
EUR 477.6

Human Rubella Virus (RV) IgG Rapid Test Kit

abx092097-20tests 20 tests
EUR 260.4

Human Rabies Virus (HRV) IgG Rapid Test Kit

abx092100-20tests 20 tests
EUR 410.4

Human Treponema Pallidum (TP) Antibody Rapid Test Kit

abx092101-20tests 20 tests
EUR 309.6

ACA (Anticardiolipin Antibody IgA) ELISA test

61 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgA)

Adv (Adenovirus Antibody IgM) ELISA test

44 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Adv (Adenovirus Antibody IgM)

ANA (Antinuclear Antibody IgG) ELISA test

52 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ANA (Antinuclear Antibody IgG)

ACA (Anticardiolipin Antibody IgM) ELISA test

60 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA (Anticardiolipin Antibody IgM)

COX (Coxsackievirus Antibody IgG) ELISA test

140 96T/Box Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgG)

COX (Coxsackievirus Antibody IgM) ELISA test

141 96T/Box Ask for price
Description: ELISA based test for quantitative detection of COX (Coxsackievirus Antibody IgM)

TOX (Toxoplasma Antibody IgG) ELISA test

32 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgG)

ACA Anticardiolipin Ab IgA ELISA test

122 96T/Box Ask for price
Description: ELISA based test for quantitative detection of ACA Anticardiolipin Ab IgA

IgE (Immune globulin E) ELISA test

21 96T/Box Ask for price
Description: ELISA based test for quantitative detection of IgE (Immune globulin)

TOX (Toxoplasma Antibody IgM) ELISA test

33 96T/Box Ask for price
Description: ELISA based test for quantitative detection of TOX (Toxoplasma Antibody IgM)

RV (Rubella Antibody IgM) ELISA test

31 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgM)

CYFRA211 (Keratin 21-1) ELISA test

23 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CYFRA211 (Keratin 21-1)

IAA (Anti-insulin antibody) ELISA test

25 96T/Box Ask for price
Description: ELISA based test for quantitative detection of IAA (Anti-insulin antibody)

RV (Rubella Antibody IgG) ELISA test

30 96T/Box Ask for price
Description: ELISA based test for quantitative detection of RV (Rubella Antibody IgG)

CMV (Cytomegalovirus Antibody IgM) ELISA test

29 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgM)

CMV (Cytomegalovirus Antibody IgG) ELISA test

28 96T/Box Ask for price
Description: ELISA based test for quantitative detection of CMV (Cytomegalovirus Antibody IgG)

PSA (Prostate-specific antigen) ELISA test

8 96T/Box Ask for price
Description: ELISA based test for quantitative detection of PSA (Prostate-specific antigen)

Syphilis anti-TP syphilis ELISA test

99 96T/Box Ask for price
Description: ELISA based test for quantitative detection of Syphilis anti-TP syphilis

vWF Acty. Kit

ABP-ACT-KIT 12 x 8 microwells
EUR 513.6

vWF Ant. Kit

ABP-TOT-KIT 12 x 8 microwells
EUR 472.8

Clenbuterol Rapid Test Kit (Colloidal gold)

abx092040-50tests 50 tests
EUR 326.4

4D Cell Biology: Adaptive optics lattice light-sheet imaging and AI powered large information processing of dwell stem cell-derived organoids

 

New strategies in stem cell 3D organoid tissue tradition, superior imaging, and large information picture analytics now permit tissue-scale 4D cell biology however at the moment out there analytical pipelines are insufficient for handing and analyzing the ensuing gigabytes and terabytes of high-content imaging information. We expressed fluorescent protein fusions of clathrin and dynamin2 at endogenous ranges in genome- edited human embryonic stem cells, which have been differentiated into intestinal epithelial organoids.

Lattice light-sheet imaging with adaptive optics (AO-LLSM) allowed us to picture massive volumes of those organoids (70 × 60 × 40 μm xyz) at 5.7 s/body. We developed an open-source information evaluation package deal termed pyLattice to course of the ensuing massive (∼60 Gb) film information units and to trace clathrin-mediated endocytosis (CME) occasions.

We then expressed fluorescent protein fusions of actin and tubulin in genome-edited induced human pluripotent stem cells, which have been differentiated into human cortical organoids. Utilizing the AO-LLSM mode on the new MOSAIC (Multimodal Optical Scope with Adaptive Imaging Correction) allowed us to picture neuronal migration deep within the organoid. We augmented pyLattice with a deep studying module and used it to course of the mind organoid information.

Widespread Sources of Irritation and Their Affect on Hematopoietic Stem Cell Biology

 

Function of assessment: Inflammatory alerts have emerged as vital regulators of hematopoietic stem cell (HSC) operate. Particularly, HSCs are extremely conscious of acute modifications in systemic irritation and this influences not solely their division price but additionally their lineage destiny. Figuring out how irritation regulates HSCs and shapes the blood system is essential to understanding the mechanisms underpinning these processes, in addition to potential hyperlinks between them.

Current findings: A widening array of physiologic and pathologic processes involving heightened irritation are actually acknowledged to critically have an effect on HSC biology and blood lineage manufacturing. Circumstances documented to have an effect on HSC operate embrace not solely acute and continual infections but additionally autoinflammatory situations, irradiation damage, and physiologic states reminiscent of ageing and weight problems.

Abstract: Recognizing the contexts throughout which irritation impacts primitive hematopoiesis is crucial to enhancing our understanding of HSC biology and informing new therapeutic interventions in opposition to maladaptive hematopoiesis that happens throughout inflammatory illnesses, infections, and cancer-related issues.

Angiostatic cues from the matrix: endothelial cell autophagy meets hyaluronan biology

The extracellular matrix encompasses a reservoir of bioactive macromolecules that modulates a cornucopia of organic features. A distinguished physique of labor posits matrix constituents as grasp regulators of autophagy and angiogenesis and gives molecular perception into how these two processes are coordinated.
Right here, we assessment present understanding of the molecular mechanisms underlying hyaluronan and its primary synthesizer, hyaluronan synthase 2 (HAS2). We critically consider the regulation and roles of soluble proteoglycans in affecting autophagy and angiogenesis.
Particularly, we assess the function of proteoglycan-evoked autophagy in regulating angiogenesis through HAS2-hyaluronan axis and ATG9A, a novel HAS2 binding associate. We talk about extracellular hyaluronan biology and the post-transcriptional and post-translational modifications that management HAS2.
We spotlight the rising group of proteoglycans that make the most of outside-in signaling to modulate autophagy and angiogenesis in most cancers microenvironments and completely assessment essentially the most up-to-date understanding of endorepellin signaling in vascular endothelia, offering perception into the temporal complexities concerned.

The Function of MicroRNAs in Improvement and Operate of Regulatory T Cells – Classes for a Higher Understanding of MicroRNA Biology

MicroRNAs (miRNAs) have emerged as vital posttranscriptional regulators of the immune system, together with operate and improvement of regulatory T (Treg) cells. Though this vital function has been firmly demonstrated via genetic fashions, key mechanisms of miRNA operate in vivo stay elusive.
Right here, we assessment the function of miRNAs in Treg cell improvement and performance. Specifically, we deal with the query what the research of miRNAs on this context reveals about miRNA biology generally, together with context-dependent operate and the function of particular person targets vs. complicated co-targeting networks.
As well as, we spotlight potential technical pitfalls and state-of-the-art approaches to enhance the mechanistic understanding of miRNA biology in a physiological context.

cDNA from Human Tumor Cell Line: MCF 7

C1255830 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

MCF 7 Membrane Lysate

XBL-10442 0.1 mg
EUR 619.8
Description: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price

Human Mcf-7 Whole Cell Lysate

LYSATE0024 200ug
EUR 180
Description: This cell lysate is prepared from human mcf-7 using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 291.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 256.8
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 342
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 232.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 248.4

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 255.6

AAV2-Luc Control Virus

AAV-320 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 2.

AAV1-Luc Control Virus

AAV-321 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 1.

AAV3-Luc Control Virus

AAV-323 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 3.

AAV4-Luc Control Virus

AAV-324 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 4.

AAV5-Luc Control Virus

AAV-325 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 5.

AAV6-Luc Control Virus

AAV-326 50 ?L
EUR 1221.6
Description: Luciferase control virus of AAV serotype 6.

Human AsPC1 / Luciferase Cell Line

SC062-Luc 2 x 106 cell/ml x 1ml
EUR 1800
Description: Firefly luciferase expression stable cell line in Human AsPC1 cells with Puromycin resistance

mouse MOPC315 / Luciferase Cell Line

SC063-Luc 2 x 106 cell/ml x 1ml
EUR 2670
Description: Firefly luciferase expression stable cell line in mouse MOPC315 cells with Puromycin resistance

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 196.8

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 153.6

A549 / Luciferase (Puromycin) stable cell line

SC043-Luc 2 x 106 cell/ml x 1ml
EUR 1104
Description: Luciferase (firefry) expression stable cell line in A549 human cancer cell line with Puromycin marker.

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 560.4

Human PANC-1 / Luciferase (Puro) Cell Line

SC068-Luc 2 x 106 cell/ml x 1ml
EUR 2670
Description: Firefly luciferase expression stable cell line in Human PANC-1 cells with Puromycin resistance

MCF-10A

C0006015 One Frozen vial
EUR 560.4

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 255.6

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

pT7- Luc

PVT10670 2 ug
EUR 361.2

pSBE- Luc

PVT10816 2 ug
EUR 361.2

pTRE3G- LUC

PVT10818 2 ug
EUR 361.2

pAP1- Luc

PVT10819 2 ug
EUR 361.2

pHSE- Luc

PVT10820 2 ug
EUR 319.2

pGRE- luc

PVT10821 2 ug
EUR 319.2

pCRE- Luc

PVT10825 2 ug
EUR 361.2

pViperin- Luc

PVT10826 2 ug
EUR 361.2

pTA- Luc

PVT10827 2 ug
EUR 361.2

pTAL- Luc

PVT10829 2 ug
EUR 361.2

pIRF3- Luc

PVT10830 2 ug
EUR 361.2

p53- Luc

PVT10836 2 ug
EUR 319.2

pBV-Luc

PVT12245 2 ug
EUR 843.6

pMR-Luc

PVT14074 2 ug
EUR 843.6

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 244.8
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line

TR860A-1 >2 x 10^6 cells
EUR 3915.6

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 200.4

pUC57-Tac-Luc

PVT18215 2 ug
EUR 309.6

p21-Luc Plasmid

PVTB00035-2c 2 ug
EUR 427.2

IgK- IFN- luc

PVT10425 2 ug
EUR 319.2

pNFAT- TA- Luc

PVT10808 2 ug
EUR 319.2

pE2F- TA- Luc

PVT10809 2 ug
EUR 319.2

pISRE- TA- Luc

PVT10810 2 ug
EUR 319.2

pGAS- TA- Luc

PVT10811 2 ug
EUR 319.2

pP53- TA- luc

PVT10814 2 ug
EUR 361.2

pStat3- TA- luc

PVT10815 2 ug
EUR 361.2

pHes1 (2.5k)- Luc

PVT10832 2 ug
EUR 319.2

pHes1 (467)- luc

PVT10833 2 ug
EUR 319.2

pNF-kB-Luc

PVT1322 2 ug
EUR 390

pGL3-ELAM-Luc

PVT14533 2 ug
EUR 718.8

proE-cad178-Luc

PVT14614 2 ug
EUR 718.8

pSynSRE-T-Luc

PVT14626 2 ug
EUR 843.6

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Lung Lysate (7 Days Old)

1402-7 0.1 mg
EUR 229.2
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)

1403-7 0.1 mg
EUR 229.2
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)

1404-7 0.1 mg
EUR 229.2
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)

1405-7 0.1 mg
EUR 229.2
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)

1406-7 0.1 mg
EUR 229.2
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)

1409-7 0.1 mg
EUR 229.2
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)

1415-7 0.1 mg
EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)

1419-7 0.1 mg
EUR 229.2
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

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