denguenaweb

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic

Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

 

Background: Propofol is a standard sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Latest research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been totally elucidated.

 

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Equipment-8 (CCK-8) to detect proliferation. Apoptosis was measured with movement cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or unfavorable management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 

small interfering ribonucleic acid (siRNA) or unfavorable management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression had been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice had been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors had been then collected from mice and analyzed by immunohistochemistry and Western blotting.

 

Outcomes: Propofol inhibited progress (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by reducing PTEN protein

 

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol therapy diminished NSCLC tumor progress (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

 

Conclusions: Our examine indicated that propofol inhibited A549 cell progress, accelerated apoptosis through the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell progress, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical therapy.

denguenaweb
denguenaweb

Expression Negative Control Lentivirus (Hygromycin)

79902-H 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Expression Negative Control Lentivirus (Puromycin)

79902-P 500 µl x 2
EUR 795
Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_

Negative Control eGFP Reporter Lentivirus

79927 500 µl x 2
EUR 835
Description: The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the control of a minimal TATA promoter, without any additional transcriptional response elements.

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1624.8

Positive control tissue section for each individua

Control-Slides-for-each-antibody Set of 25
EUR 355
Description: Positive control tissue section for each individual antibody; Based on availability; INQUIRE

Lenti-HOXA10-GFP Lentivirus

LV646 10 ml
EUR 973.2

Lenti-HOXB8-GFP Lentivirus

LV634 10 ml
EUR 973.2

CD14-Rluc (GFP) Lentivirus 

LVP1000-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Bsd) Lentivirus 

LVP1001-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Neo) Lentivirus 

LVP1001-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (Puro) Lentivirus 

LVP1001-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-GFP (RFP) Lentivirus 

LVP1001-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-RFP (GFP) Lentivirus 

LVP1002-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (GFP) Lentivirus 

LVP1003-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Rluc (GFP) Lentivirus 

LVP1004-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Bsd) Lentivirus 

LVP1005-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Neo) Lentivirus 

LVP1005-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (Puro) Lentivirus 

LVP1005-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-GFP (RFP) Lentivirus 

LVP1005-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-RFP (GFP) Lentivirus 

LVP1006-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (GFP) Lentivirus 

LVP1007-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Rluc (GFP) Lentivirus 

LVP1008-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Bsd) Lentivirus 

LVP1009-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Neo) Lentivirus 

LVP1009-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (Puro) Lentivirus 

LVP1009-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-GFP (RFP) Lentivirus 

LVP1009-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-RFP (GFP) Lentivirus 

LVP1010-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Luc (GFP) Lentivirus 

LVP1011-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD68-Rluc (GFP) Lentivirus 

LVP1012-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Bsd) Lentivirus 

LVP1013-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Neo) Lentivirus 

LVP1013-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (Puro) Lentivirus 

LVP1013-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-GFP (RFP) Lentivirus 

LVP1013-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-RFP (GFP) Lentivirus 

LVP1014-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Luc (GFP) Lentivirus 

LVP1015-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Flt1-Rluc (GFP) Lentivirus 

LVP1016-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Bsd) Lentivirus 

LVP1017-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Neo) Lentivirus 

LVP1017-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (Puro) Lentivirus 

LVP1017-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-GFP (RFP) Lentivirus 

LVP1017-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-RFP (GFP) Lentivirus 

LVP1018-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-Luc (GFP) Lentivirus 

LVP1019-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ICAM2-Rluc (GFP) Lentivirus 

LVP1020-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under the human ICAM-2's promoter which expressed specifically in vasculature endothelial cells and megakaryocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Bsd) Lentivirus 

LVP1021-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Neo) Lentivirus 

LVP1021-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (Puro) Lentivirus 

LVP1021-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-GFP (RFP) Lentivirus 

LVP1021-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-RFP (GFP) Lentivirus 

LVP1022-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Luc (GFP) Lentivirus 

LVP1023-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

MB-Rluc (GFP) Lentivirus 

LVP1024-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Bsd) Lentivirus 

LVP1025-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Neo) Lentivirus 

LVP1025-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (Puro) Lentivirus 

LVP1025-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-GFP (RFP) Lentivirus 

LVP1025-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-RFP (GFP) Lentivirus 

LVP1026-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Luc (GFP) Lentivirus 

LVP1027-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SPB-Rluc (GFP) Lentivirus 

LVP1028-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Bsd) Lentivirus 

LVP1029-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Neo) Lentivirus 

LVP1029-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (Puro) Lentivirus 

LVP1029-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-GFP (RFP) Lentivirus 

LVP1029-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-RFP (GFP) Lentivirus 

LVP1030-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-Luc (GFP) Lentivirus 

LVP1031-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

SYN1-Rluc (GFP) Lentivirus 

LVP1032-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Bsd) Lentivirus 

LVP1033-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Neo) Lentivirus 

LVP1033-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (Puro) Lentivirus 

LVP1033-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-GFP (RFP) Lentivirus 

LVP1033-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-RFP (GFP) Lentivirus 

LVP1034-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Luc (GFP) Lentivirus 

LVP1035-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

aFP-Rluc (GFP) Lentivirus 

LVP1036-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Bsd) Lentivirus 

LVP1037-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Neo) Lentivirus 

LVP1037-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (Puro) Lentivirus 

LVP1037-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-GFP (RFP) Lentivirus 

LVP1037-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-RFP (GFP) Lentivirus 

LVP1038-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-Luc (GFP) Lentivirus 

LVP1039-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CCKAR-Rluc (GFP) Lentivirus 

LVP1040-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human CCKR's promoter which is predominantly expressed in human pancreatic cancer. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Bsd) Lentivirus 

LVP1041-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Neo) Lentivirus 

LVP1041-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (Puro) Lentivirus 

LVP1041-P 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-GFP (RFP) Lentivirus 

LVP1041-R 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-RFP (GFP) Lentivirus 

LVP1042-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Luc (GFP) Lentivirus 

LVP1043-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

HE4-Rluc (GFP) Lentivirus 

LVP1044-G 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express Renilla luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (Bsd) Lentivirus 

LVP1045-B 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

PSA-GFP (Neo) Lentivirus 

LVP1045-N 1x107 IFU/ml x 200ul
EUR 662.4
Description: Pre-made lentivirus express GFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

 

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

 

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely mirror human biology and the pathophysiology of human illness. Right here we report a sturdy, reproducible, and scalable protocol for the era of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing brief publicity to nonengineered matrices.

These hepatic organoids comply with outlined levels of hepatic growth and categorical increased ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to numerous metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs underneath each 2D tradition and three-dimensional (3D) organoid situations. Beneath typical 2D tradition situations, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects had been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the purposeful interrogation of genes recognized via human genetic investigation.

The epitranscriptome in stem cell biology and neural growth

The blossoming discipline of epitranscriptomics has not too long ago garnered consideration throughout many fields by findings that chemical modifications on RNA have immense organic penalties. Methylation of nucleotides in RNA, together with N6-methyladenosine (m6A), 2-O-dimethyladenosine (m6Am), N1-methyladenosine (m1A), 5-methylcytosine (m5C), and isomerization of uracil to pseudouridine (Ψ), have the potential to change RNA processing occasions and contribute to developmental processes and completely different illnesses.

 

Although the abundance and roles of some RNA modifications stay contentious, the epitranscriptome is regarded as particularly related in stem cell biology and neurobiology. Specifically, m6A happens on the highest ranges within the mind and performs main roles in embryonic stem cell differentiation, mind growth, and neurodevelopmental problems.

Nevertheless, research in these areas have reported conflicting outcomes on epitranscriptomic regulation of stem cell pluripotency and mechanisms in neural growth. On this assessment we offer an outline of the present understanding of a number of RNA modifications and disentangle the varied findings on epitranscriptomic regulation of stem cell biology and neural growth.

Ciliate conduct: blueprints for dynamic cell biology and microscale robotics

Place a drop of pond water underneath the microscope, and you’ll possible discover an ocean of extraordinary and various single-celled organisms known as ciliates. This exceptional group of single-celled organisms wields microtubules, lively methods, electrical signaling, and chemical sensors to construct intricate geometrical buildings and carry out advanced behaviors that may seem indistinguishable from these of macroscopic animals.
Advances in pc imaginative and prescient and machine studying are making it potential to fully digitize and monitor the dynamics of advanced ciliates and mine these knowledge for the hidden construction, patterns, and motifs which are answerable for their behaviors.
By deconstructing the variety of ciliate behaviors within the pure world, themes for organizing and controlling matter on the microscale are starting to take maintain, suggesting new modular approaches for the design of autonomous molecular machines that emulate nature’s best examples.

Sarcomatoid renal cell carcinoma: biology, pure historical past and administration

Sarcomatoid dedifferentiation is an unusual characteristic that may happen in most histological subtypes of renal cell carcinomas (RCCs) and carries a decidedly poor prognosis. Traditionally, typical therapies for sarcomatoid RCCs (sRCCs) have proven little efficacy, and median survival is usually 6-13 months.
Regardless of being first described in 1968, the mechanisms driving sarcomatoid dedifferentiation stay poorly understood, and data and therapy choices obtainable to physicians and sufferers are restricted. When recognized at an early stage, surgical intervention stays the therapy of alternative.
Nevertheless, preoperative identification via routine imaging or biopsy is unreliable and most sufferers current with superior illness and systemic signs. For these sufferers, the function of cytoreductive nephrectomy is disputed.
The growth of immunotherapies authorised for RCCs has generated a seek for biomarkers that could be indicative of therapy response in sRCCs, though a confirmed efficient systemic agent stays elusive.
PDL1 expression is elevated in sarcomatoid dedifferentiated renal tumours, which means that sufferers with sRCCs may benefit from PD1 and/or PDL1 immune checkpoint blockade remedy. Therapy outcomes for sarcomatoid tumours have remained comparatively constant in contrast with different RCCs, however additional investigation of the tumour-immune cell microenvironment may yield insights into additional therapeutic potentialities.

 

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 232.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100 each
EUR 248.4

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 255.6

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 196.8

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 153.6

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 560.4

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

MCF-10A

C0006015 One Frozen vial
EUR 560.4

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 255.6

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1624.8

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1624.8

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2799.6

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2799.6

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2799.6

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2799.6

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2799.6

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1672.8

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1672.8

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1672.8

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1672.8

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1672.8

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2799.6

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2799.6

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2799.6

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2799.6

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2799.6

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2799.6

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2799.6

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2799.6

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2799.6

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 244.8
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 200.4

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 854.4

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Lung Lysate (7 Days Old)

1402-7 0.1 mg
EUR 229.2
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)

1403-7 0.1 mg
EUR 229.2
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)

1404-7 0.1 mg
EUR 229.2
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)

1405-7 0.1 mg
EUR 229.2
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)

1406-7 0.1 mg
EUR 229.2
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)

1409-7 0.1 mg
EUR 229.2
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)

1415-7 0.1 mg
EUR 229.2
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)

1419-7 0.1 mg
EUR 229.2
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)

1420-7 0.1 mg
EUR 229.2
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

ICP Quality Control Standard 7

QC-7 125ML
EUR 176.7

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Bone Morphogenetic protein-7

7-00184 2µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00185 10µg Ask for price

Recombinant Human Bone Morphogenetic protein-7

7-00186 1mg Ask for price

Recombinant Human Galectin-7 His Tag

7-00445 5µg Ask for price

Recombinant Human Galectin-7 His Tag

7-00446 20µg Ask for price

Recombinant Human Galectin-7 His Tag

7-00447 1mg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00901 2µg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00902 10µg Ask for price

Recombinant Human Interleukin-7, His Tag

7-00903 1mg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04639 1µg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04640 5µg Ask for price

Recombinant Human Calcium Binding Protein 7

7-04641 50µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05707 5µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05708 25µg Ask for price

Recombinant Human Nuclear Import 7 Homolog

7-05709 1mg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05800 5µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05801 20µg Ask for price

Recombinant Human Parkinson Disease Protein 7

7-05802 1mg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07543 100µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07544 500µg Ask for price

Recombinant Toxoplasma Gondii P29 (GRA 7)

7-07545 1000µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase

7-03547 /2µg Ask for price

Recombinant Human RNA (guanine-7-) Methyltransferase

7-03548 10µg Ask for price

Leave a Reply

Your email address will not be published.