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Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic

Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo

 

Background: Propofol is a standard sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Latest research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been totally elucidated.

 

Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Equipment-8 (CCK-8) to detect proliferation. Apoptosis was measured with movement cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or unfavorable management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN)

 

small interfering ribonucleic acid (siRNA) or unfavorable management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression had been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR). In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice had been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors had been then collected from mice and analyzed by immunohistochemistry and Western blotting.

 

Outcomes: Propofol inhibited progress (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by reducing PTEN protein

 

expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol therapy diminished NSCLC tumor progress (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).

 

Conclusions: Our examine indicated that propofol inhibited A549 cell progress, accelerated apoptosis through the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell progress, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical therapy.

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GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354

CamKII-Luc (GFP) Lentivirus 

LVP1055-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human CamKII's promoter which restrictly expressed in neurons of the neocortex and hippocampus, also pyramidal neurons. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CamKII-Rluc (GFP) Lentivirus 

LVP1056-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human CamKII's promoter which restrictly expressed in neurons of the neocortex and hippocampus, also pyramidal neurons. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-GFP (Bsd) Lentivirus 

LVP1104-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-GFP (Bsd) Lentivirus 

LVP1104-B-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-GFP (Neo) Lentivirus 

LVP1104-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-GFP (Neo) Lentivirus 

LVP1104-N-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-GFP (Puro) Lentivirus 

LVP1104-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-GFP (Puro) Lentivirus 

LVP1104-P-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-GFP (RFP) Lentivirus 

LVP1104-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-GFP (RFP) Lentivirus 

LVP1104-R-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-RFP (GFP) Lentivirus 

LVP1105-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-RFP (GFP) Lentivirus 

LVP1105-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-Luc (GFP) Lentivirus 

LVP1106-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-Luc (GFP) Lentivirus 

LVP1106-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.

Nephrin-Rluc (GFP) Lentivirus 

LVP1107-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Nephrin-Rluc (GFP) Lentivirus 

LVP1107-G-PBS 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under human Nephrin's promoter which is specifically expressed in human kidney podocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

EGR1-GFP (Bsd) Lentivirus 

LVP1119-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-GFP (Bsd) Lentivirus 

LVP1119-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Blasticidin selection marker under RSV promoter, provided in PBS solution.

EGR1-GFP (Neo) Lentivirus 

LVP1119-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-GFP (Neo) Lentivirus 

LVP1119-N-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the Neomycin selection marker under RSV promoter, provided in PBS solution.

EGR1-GFP (Puro) Lentivirus 

LVP1119-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the early growth response gene (EGR1). This lentivirus contains the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-GFP (Puro) Lentivirus 

LVP1119-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the early growth response gene (EGR1). This lentivirus contains the puromycin selection marker under RSV promoter, provided in PBS solution.

EGR1-GFP (RFP) Lentivirus 

LVP1119-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker RFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-GFP (RFP) Lentivirus 

LVP1119-R-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker RFP under RSV promoter, provided in PBS solution.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-RFP (GFP) Lentivirus 

LVP1120-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains another fluorescent marker GFP under RSV promoter, provided in PBS solution.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-Luc (GFP) Lentivirus 

LVP1121-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.

EGR1-Rluc (GFP) Lentivirus 

LVP1122-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EGR1-Rluc (GFP) Lentivirus 

LVP1122-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla Luciferase reporter under the promoter of human early growth response gene-1 (EGR1). This lentivirus contains the fluorescent marker GFP under RSV promoter, provided in PBS solution.

GFAP-GFP (Bsd) Lentivirus 

LVP1125-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-GFP (Bsd) Lentivirus 

LVP1125-B-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Blasticidin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-GFP (Neo) Lentivirus 

LVP1125-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-GFP (Neo) Lentivirus 

LVP1125-N-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the Neomycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-GFP (Puro) Lentivirus 

LVP1125-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-GFP (Puro) Lentivirus 

LVP1125-P-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the puromycin selection marker under RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-GFP (RFP) Lentivirus 

LVP1125-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-GFP (RFP) Lentivirus 

LVP1125-R-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express GFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-RFP (GFP) Lentivirus 

LVP1126-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express RFP reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-Luc (GFP) Lentivirus 

LVP1127-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Firefly luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter. Lentivirus concentrated and provided in PBS solution.

GFAP-Rluc (GFP) Lentivirus 

LVP1128-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

GFAP-Rluc (GFP) Lentivirus 

LVP1128-G-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Pre-made lentivirus express Renilla luciferase reporter under human GFAP's promoter which expressed in astrocytes in Central Nervous System. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter. Lentivirus concentrated and provided in PBS solution.

EBNA1 (GFP-Puro) Lentivirus

LVP1134-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA1 gene under EF1a promoter, containing GFP-Puromycin dual marker.

EBNA2 (GFP-Puro) Lentivirus

LVP1135-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Epstein Barr Virus' EBNA2 gene under EF1a promoter, containing GFP-Puromycin dual marker.

E1A (GFP-Puro) Lentivirus

LVP1137-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing Adenovirus type 5's E1A gene under EF1a promoter, containing GFP-Puromycin dual marker.

HOXA9 (GFP-Puro) Lentivirus

LVP1138-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human HOXA9 gene under EF1a promoter, containing GFP-Puromycin dual marker.

KRas_G12V (GFP-Puro) Lentivirus

LVP1139-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human Kras V12G mutant under EF1a promoter, containing GFP-Puromycin dual marker.

CDK4 (GFP-Puro) Lentivirus

LVP1140-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human CDK4 gene under EF1a promoter, containing GFP-Puromycin dual marker.

cMyc (GFP-Puro) Lentivirus

LVP1141-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human cMyc gene under EF1a promoter, containing GFP-Puromycin dual marker.

AR-GFP (Bsd) Lentivirus

LVP912-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-GFP (Neo) Lentivirus  

LVP912-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-GFP (Puro) Lentivirus

LVP912-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made Lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This Lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-GFP (RFP) Lentivirus 

LVP912-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-RFP (GFP) Lentivirus 

LVP913-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Luc (GFP) Lentivirus 

LVP914-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AR-Rluc (GFP) Lentivirus 

LVP915-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-GFP (Bsd) Lentivirus 

LVP937-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-GFP (Neo) Lentivirus 

LVP937-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-GFP (Puro) Lentivirus 

LVP937-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-GFP (RFP) Lentivirus 

LVP937-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-RFP (GFP) Lentivirus 

LVP938-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-Luc (GFP) Lentivirus 

LVP939-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

ISRE-Rluc (GFP) Lentivirus 

LVP940-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-GFP (Bsd) Lentivirus 

LVP941-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-GFP (Neo) Lentivirus 

LVP941-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-GFP (Puro) Lentivirus 

LVP941-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-GFP (RFP) Lentivirus 

LVP941-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-RFP (GFP) Lentivirus 

LVP942-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-Luc (GFP) Lentivirus 

LVP943-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CRE_TRE-Rluc (GFP) Lentivirus 

LVP944-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-GFP (Bsd) Lentivirus 

LVP945-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-GFP (Neo) Lentivirus 

LVP945-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-GFP (Puro) Lentivirus 

LVP945-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-GFP (RFP) Lentivirus 

LVP945-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-RFP (GFP) Lentivirus 

LVP946-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Luc (GFP) Lentivirus 

LVP947-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

Gli-Rluc (GFP) Lentivirus 

LVP948-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-GFP (Bsd) Lentivirus 

LVP949-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-GFP (Neo) Lentivirus 

LVP949-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-GFP (Puro) Lentivirus 

LVP949-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-GFP (RFP) Lentivirus 

LVP949-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-RFP (GFP) Lentivirus 

LVP950-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Luc (GFP) Lentivirus 

LVP951-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

G_RE-Rluc (GFP) Lentivirus 

LVP952-G 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-GFP (Bsd) Lentivirus 

LVP953-B 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-GFP (Neo) Lentivirus 

LVP953-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

AP1-GFP (Puro) Lentivirus 

LVP953-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express GFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

 

Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene

 

Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely mirror human biology and the pathophysiology of human illness. Right here we report a sturdy, reproducible, and scalable protocol for the era of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing brief publicity to nonengineered matrices.

These hepatic organoids comply with outlined levels of hepatic growth and categorical increased ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to numerous metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.

We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs underneath each 2D tradition and three-dimensional (3D) organoid situations. Beneath typical 2D tradition situations, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.

In distinction, when cultured as 3D hepatic organoids, the differentiation defects had been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the purposeful interrogation of genes recognized via human genetic investigation.

The epitranscriptome in stem cell biology and neural growth

The blossoming discipline of epitranscriptomics has not too long ago garnered consideration throughout many fields by findings that chemical modifications on RNA have immense organic penalties. Methylation of nucleotides in RNA, together with N6-methyladenosine (m6A), 2-O-dimethyladenosine (m6Am), N1-methyladenosine (m1A), 5-methylcytosine (m5C), and isomerization of uracil to pseudouridine (Ψ), have the potential to change RNA processing occasions and contribute to developmental processes and completely different illnesses.

 

Although the abundance and roles of some RNA modifications stay contentious, the epitranscriptome is regarded as particularly related in stem cell biology and neurobiology. Specifically, m6A happens on the highest ranges within the mind and performs main roles in embryonic stem cell differentiation, mind growth, and neurodevelopmental problems.

Nevertheless, research in these areas have reported conflicting outcomes on epitranscriptomic regulation of stem cell pluripotency and mechanisms in neural growth. On this assessment we offer an outline of the present understanding of a number of RNA modifications and disentangle the varied findings on epitranscriptomic regulation of stem cell biology and neural growth.

Ciliate conduct: blueprints for dynamic cell biology and microscale robotics

Place a drop of pond water underneath the microscope, and you’ll possible discover an ocean of extraordinary and various single-celled organisms known as ciliates. This exceptional group of single-celled organisms wields microtubules, lively methods, electrical signaling, and chemical sensors to construct intricate geometrical buildings and carry out advanced behaviors that may seem indistinguishable from these of macroscopic animals.
Advances in pc imaginative and prescient and machine studying are making it potential to fully digitize and monitor the dynamics of advanced ciliates and mine these knowledge for the hidden construction, patterns, and motifs which are answerable for their behaviors.
By deconstructing the variety of ciliate behaviors within the pure world, themes for organizing and controlling matter on the microscale are starting to take maintain, suggesting new modular approaches for the design of autonomous molecular machines that emulate nature’s best examples.

Sarcomatoid renal cell carcinoma: biology, pure historical past and administration

Sarcomatoid dedifferentiation is an unusual characteristic that may happen in most histological subtypes of renal cell carcinomas (RCCs) and carries a decidedly poor prognosis. Traditionally, typical therapies for sarcomatoid RCCs (sRCCs) have proven little efficacy, and median survival is usually 6-13 months.
Regardless of being first described in 1968, the mechanisms driving sarcomatoid dedifferentiation stay poorly understood, and data and therapy choices obtainable to physicians and sufferers are restricted. When recognized at an early stage, surgical intervention stays the therapy of alternative.
Nevertheless, preoperative identification via routine imaging or biopsy is unreliable and most sufferers current with superior illness and systemic signs. For these sufferers, the function of cytoreductive nephrectomy is disputed.
The growth of immunotherapies authorised for RCCs has generated a seek for biomarkers that could be indicative of therapy response in sRCCs, though a confirmed efficient systemic agent stays elusive.
PDL1 expression is elevated in sarcomatoid dedifferentiated renal tumours, which means that sufferers with sRCCs may benefit from PD1 and/or PDL1 immune checkpoint blockade remedy. Therapy outcomes for sarcomatoid tumours have remained comparatively constant in contrast with different RCCs, however additional investigation of the tumour-immune cell microenvironment may yield insights into additional therapeutic potentialities.

 

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 207

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 213

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 164

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 128

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 467

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1354

MCF-10A

C0006015 One Frozen vial
EUR 467

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 213

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2333

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2333

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2333

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2333

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2333

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2333

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2333

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2333

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1394

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1394

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1394

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1394

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1394

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2333

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2333

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2333

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2333

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2333

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2333

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 712

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03154 5µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03155 20µg Ask for price

Recombinant Human Matrix Metalloproteinase-7

7-03156 1mg Ask for price

Lung Lysate (7 Days Old)

1402-7 0.1 mg
EUR 191
Description: Lung tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Brain Lysate (7 Days Old)

1403-7 0.1 mg
EUR 191
Description: Brain tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Liver Lysate (7 Days Old)

1404-7 0.1 mg
EUR 191
Description: Liver tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (7 Days Old)

1405-7 0.1 mg
EUR 191
Description: Kidney tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Spleen Lysate (7 Days Old)

1406-7 0.1 mg
EUR 191
Description: Spleen tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Thymus Lysate (7 Days Old)

1409-7 0.1 mg
EUR 191
Description: Thymus tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Stomach Lysate (7 Day Old)

1415-7 0.1 mg
EUR 191
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Skin Lysate (7 Days Old)

1419-7 0.1 mg
EUR 191
Description: Skin tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Eye Lysate (7 Days Old)

1420-7 0.1 mg
EUR 191
Description: Eye tissue lysate (7 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

GFP Lentivirus Control

LTV-300 1 vial
EUR 566
Description: HIV-1 Lentivirus

GFP Recombinant Adenovirus

ADV-004 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the GFP gene

GFP Quantitation Kit

AKR-120 100 assays
EUR 577
Description: Cell Biolabs? GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.

GFP ELISA Kit

AKR-121 96 assays
EUR 734
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

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